Biotinylated mouse tim4-fc

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August undefined, 2024

Weblipids, including PtdCho, PtdEtn, and sphingomyelin. Tim4–Fc, a fusion protein of the extracellular region of mTim4 and hu-man IgG1–Fc (12), showed a similar specificity for PtdSer, with a three to eight times stronger affinity than that of mProS or mGas6 for PtdSer (Fig. 1). Interaction Between Mouse TAM Ligands and Their Receptors. We WebMar 12, 2014 · TIM4 is the main receptor mediating the phagocytosis of PtdSer-bearing targets in murine macrophages. Bone marrow-derived macrophages (BMDMs) from TIM4 − / − mice were reported to have a reduced ability to engulf apoptotic bodies (Martinez et al., 2011).This observation suggests that TIM4 may be the primary PtdSer receptor in these …

BV421 Rat Anti-Mouse TIM-4 - BD Biosciences

WebMeaning of biotinylated. What does biotinylated mean? Information and translations of biotinylated in the most comprehensive dictionary definitions resource on the web. WebMar 27, 2005 · The newly identified TIM family of proteins is associated with regulation of T helper type 1 (TH1) and TH2 immune responses. TIM-1 is genetically linked to asthma and is a receptor for hepatitis A ... how intraoral scanners work

Title Synergistic effect of Tim4 and MFG-E8 null mutations on …

WebMonoclonal Hamster‑Armenian anti‑Mouse TIMD4 / TIM4 / TIM‑4 Antibody (FITC, IF) LS‑C663029. ... FC: Cells were incubated on ice for 30 min with 1 ug/ml biotinylated Kat5-18 in 100 ul staining solution (PBS containing 2% FCS), followed by incubation with 2 ug/ml Allophycocyanin (APC)-Cyanine 7 (Cy7)-conjugated streptavidin and 2 ug/ml ... WebFeb 9, 2024 · Needle-like multi-walled carbon nanotubes (MWCNTs) are engulfed by macrophages, which cause inflammation and asbestosis-like pathogenesis in rodents; however, how macrophages recognize … how intricate can the cricut cut

Identification of Tim4 as a phosphatidylserine receptor

Anti-mouse IgG (H+L), Biotinylated Antibody #14709

WebFUJIFILM biotinylated mouse tim4 fc Biotinylated Mouse Tim4 Fc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … WebApr 9, 2024 · Moderate. Difficult. Very difficult. Pronunciation of biotinylated with 1 audio pronunciations. 1 rating. high heels shoes uk manufacturerWebFeb 9, 2024 · Tim4 is involved in recognition of MWCNTs by mouse peritoneal macrophages. (A) Recognition of MWCNTs (30 μg/mL) by WT or Tim4−/− mouse … how intimidating are you

"WebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, " - Biotinylated mouse tim4-fc

Biotinylated mouse tim4-fc

Mouse macrophages show different requirements for ... - PNAS

WebTim4 alone did not support efferocytosis, but enhanced TAM-dependent efferocytosis. Resident peritoneal macrophages, Kupffer cells, and CD169+ skin macrophages … WebAlternative Name: TIMD-4, SMUCKLER, Tim4, Timd4 Reactivity: Mouse (Tested in Development) Isotype: Rat LEW, also known as Lewis IgG1, κ ... Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO. Size 0.1 mg Cat No. 553141. Stain Buffer (FBS) RUO. Size 500 mL Cat No. 554656. Stain Buffer (BSA) RUO. Size 500 mL Cat No. 554657.

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WebTim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 … WebJun 29, 2024 · An ELISA plate (AGC Techno Glass, Shizuoka, Japan) was coated with 1 μg/mL recombinant mouse TIM4-Fc protein (FUJIFILM Wako Pure Chemical)/50 mM carbonate-bicarbonate buffer (pH 9.6) and ...

WebCD45 in Mouse Thymus. CD45 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # … WebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 …

WebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. WebThe TIM4-affinity isolation method helps overcome the disadvantages of the affinity isolation method and enables the isolation of heterogeneous SEVs at high purity. This …

WebTIM-4 (T cell; immunoglobulin; Mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIM-4 is exclusively expressed on antigen-presenting cells, where it mediates phagocytosis of apoptotic cells and plays an important role in maintaining tolerance. TIM-4 binds specifically to TIM-1 which is also ...

WebApr 1, 2024 · Exosome concentration by ultracentrifugation and Tim4 affinity columns We also concentrated Exo by the Tim4-affinity method using a MagCapture Exosome Isolation Kit PS (Wako, Japan). Streptavidin magnetic beads (0.6 mg), bound with 1 μg of biotinylated mouse Tim4-Fc, were added to precleared CSs supplemented with 2 mM … howin tradingWebThe cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining ... how intimidating are you quizWebTIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by … how intimate is dancingWebWe offer Anti-Mouse secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep. JIR secondary antibodies are available as whole IgG and F (ab') 2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple blocking and labeling protocols. how in touch with your emotions are youWebAug 31, 2024 · The EVs were isolated from the sera of PC patients or NC in cohort-1 using the Magcapture Exosome Isolation Kit (Fujifilm) according to the manufacturer’s protocol. In brief, 0.6 mg of streptavidin magnetic beads, bound with 1 µg of biotinylated mouse Tim4-Fc, was added to the serum supplement and rotated overnight at 4 °C. how intj achieve potentialWebNov 10, 2024 · Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified) FCM-M82W5: HEK293: Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, Avitag™ (MALS & BLI verified) FCM-M82W6 ... how introduce key cod minecraftWebMouse TIM-4 Antibody Summary Species Reactivity Mouse Specificity Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with … how intricate is the human body