Cfos ttaマウス
WebNov 10, 2024 · 为此,LifeCanvas现在进一步对其技术进行更新,以使整个组织中c-FOS蛋白的均匀标记成为可能。. 这将支持神经科学家进行更广泛的研究,包括与疾病和年龄相关的对外部刺激的神经反应变化,学习和记忆的研究,检测脑肿瘤并通过整脑的3D研究追踪神经发 … WebJul 12, 2024 · The two viruses required for activity-tagging, AAV-P cfos-tTA and AAV-P TRE-tight-hM3D q-mCherry (or AAV-P TRE-tight-flex-hM3D q-mCherry), were mixed in a 1:1 ratio. Viral infusions, using either a steel injector (10 μl-Hamilton #701) or a silica micro-capillary made in house, were performed with an electronic pump and optimized for the …
Cfos ttaマウス
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WebPlasmid pAAV-cFos-tTA-pA from Dr. William Wisden's lab contains the insert cfos promoter linked to the tet activator gene and is published in Nat Neurosci. 2015 Apr;18(4):553-61. … Webcfos-tTA x tetO-TeNT-GFPマウスを用いることにより、恐怖条件付け学習時に活動した神経 細胞集団のシナプス伝達を選択的かつ一時的に遮断し、その記憶の表出に与える影響を行 動学的に解析した(図3)。具体的には、Doxycycline(Dox)を含まない餌を与え始めて3 …
WebJan 7, 2024 · home > Addgene > pAAV-cFos-tTA-pA product summary. company name : Addgene. product type : cDNA. product name : pAAV-cFos-tTA-pA. catalog : 66794. more info or order : Addgene product webpage. citations: 3. Reference; Poll S, Mittag M, Musacchio F, Justus L, Giovannetti E, Steffen J, et al. Memory trace interference impairs … WebSep 26, 2024 · The cfos-tTA strategy couples the c-Fos. promoter to the tetracycline transactivator (tTA), which, in its. protein form, directly binds to the tetracycline response element
Webc-fosプロモーター活性依存的にチャネルロドプシンを発現するトランスジェニックマウス (cFos-pro-tTA x TRE ChR2-YFPマウス)にtrainingとして電気ショック(稲妻印)を与え … WebFeb 23, 2015 · The TetTag transgene components, cFos-tTA and P TRE-tight-hM 3 D q-mCHERRY, were placed into two separate AAV transgenes. As a building block, we …
Web008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible …
WebApr 4, 2024 · Tg (Fos-tTA)1Mmay. Mutation details : The c-fos-tTA transgene was designed with the promoter region from the mouse FBJ osteosarcoma oncogene (Fos or c-fos; at … federal estate tax proposed changesWebApr 1, 2011 · そこでcfos-tTA x tetO-hM3Dqダブルトランスジェニックマウスを用いてDox非存在条件下において恐怖条件付け学習課題を行うことにより学習課題時に活動した神経細胞集団にhM3Dqの発現による標識を行った。 federal estate tax exemption clawbackWeb018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J These cfos-htTA mice (cfos-tTA/cfos-shEGFP) have two co-injected transgenes, cfos-tTA and cfos-shEGFP, resulting in … federal estate tax return filing thresholdWebApr 4, 2024 · Tg (Fos-tTA)1Mmay. Mutation details : The c-fos-tTA transgene was designed with the promoter region from the mouse FBJ osteosarcoma oncogene (Fos or c-fos; at least 611 bp of 5' untranscribed sequence that encompasses the known mouse c-fos regulatory elements) placed upstream of the tetracycline-regulated transactivator gene … decorating ideas bathroom mirrors shelvesWeb具体的には、申請者らが開発したcfos-tTAとtetO-taulacZのダブルトランスジェニックマウスをを用いて文脈依存的恐怖条件付けを行い、この学習刺激により活動した神経細胞集団をtau-lacZで標識した。2日間のDox入りの餌での飼育後、同じマウスを再び同じ装置に3 ... decorating ideas beach themeWebDec 11, 2024 · The hippocampus is involved in processing a variety of mnemonic computations specifically the spatiotemporal components and emotional dimensions of contextual memory. 1–3 Recent studies have... federal estimated income tax formsWebMar 15, 2024 · Transgenic cfos-tTA/tetO-ChEF mice (TG) were fear-conditioned in the absence of doxycycline to allow ChEF expression in RSC neurons that were activated … decorating ideas beach house