High-speed counter-current chromatography

WebOct 7, 2024 · The high-speed counter-current chromatography (HSCCC) was implemented by employing a two-phase solvent system which consist of n-hexane–ethyl acetate–methanol-water (4:5:4:5, v/v/v/v) and n-hexane–ethyl acetate–methanol-water (1:5:1:5, v/v/v/v) to separate flavonoid into five fractions. WebOct 16, 2013 · High-speed counter-current chromatography (HSCCC) was applied for the first time for the preparative separation of spirobisnaphthalenes from a crude extract of the endophytic fungus Berkleasmium sp. Dzf12, associated with the medicinal plant Dioscorea zingiberensis. Six spirobisnaphthalenes were successfully separated by HSCCC with a …

Separation and purification of astaxanthin from Phaffia …

WebHigh-speed counter-current chromatography yielded, from 108 mg of the partially purified extract, 53 mg rutin and 32 mg nicotiflorin with 92.5% and 92.2% recovery, with each at over 96.5% purity by high-performance liquid chromatography analysis. Their structures were identified by 1H NMR and 13C NMR. MeSH terms Chromatography, High Pressure Liquid WebJul 29, 1999 · Covering definitions, concepts, and applications, Countercurrent Chromatography recounts the developments in two types of liquid-liquid chromatography termed countercurrent-high-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC)-as well as the HSCCC-derived cross-axis CCC, … on the links magazine https://cashmanrealestate.com

Countercurrent chromatography - Wikipedia

WebApr 18, 2024 · Moreover, an off-line hyphenated method combining high-speed counter-current chromatography, affinity-based ultrafiltration, and liquid chromatography–tandem mass spectrometry was used to identify and characterize the DCR compounds with tyrosinase inhibitory activity. Our results indicated that, DCR significantly decreased the … WebOct 3, 2024 · High speed counter current chromatography (HSCCC) utilizes a liquid stationary-phase and, hence, does not suffer from irretrievable adsorption associated … WebHigh speed counter-current chromatography (HSCCC), a liquid–liquid separation chromatographic technology, has been widely applied for the large-scale separation of chemical compositions and biotransformation reactor. 14,15 It provides unique advantages such as lager loading capacity and higher sample recovery compared to traditional … on the list china

Preparative separation and purification of squalene from the …

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High-speed counter-current chromatography

Preparative separation and purification of squalene from the …

WebNov 7, 2024 · Finally, the targets were introduced to preparative high-speed counter-current chromatography for separation using ethyl acetate/n-butanol/acetic acid/water (2/7/1/10, v/v/v/v) solvent system. As a result, 191 mg of Mucic acid 1,4-lactone 5-O-gallate, 370 mg of β-Glucogallin, 301 mg of Gallic acid, 195 mg of Mucic acid 1,4-lactone 3-O-gallate ... WebAll-liquid chromatography methods such as countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) have been widely used for the isolation of natural target compounds from complex plant and food samples [19,20,21,22]. CPC uses two non-miscible liquids as the stationary phase and mobile phase.

High-speed counter-current chromatography

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WebHigh speed counter current chromatography (HSCCC) utilizes a liquid stationary-phase and, hence, does not suffer from irretrievable adsorption associated with conventional chromatography procedures. It is a good alternative for such techniques because of its … Webmachines were collectively known as high speed countercurrent chromatograms (hsccc). This breakthrough delivered two significant aspects of instrument performance: 1. Firstly, separation time was reduced by a factor of 10, so now these high capacity separations could be performed in times similar to solid phase chromatography, such as HPLC or ...

WebCounter-current chromatography (CCC), frequently referred to as high-speed counter-current chromatography (HSCCC, see also below), is a separation technique based on the … WebJul 26, 2012 · A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two …

WebJournal of Chromatography B Volumes 1029–1030 , 1 September 2016 , Pages 191-197 Separation and purification of astaxanthin from Phaffia rhodozyma by preparative high … WebHere, we proposed a high-speed countercurrent chromatography (HSCCC)-based method to recover extracted compounds and DES. As a proof of concept, the chrysanthemum DES …

WebOct 26, 2009 · The development of HPCCC instruments combined with the limitations that chemists are experiencing with solid/liquid chromatography techniques, has created an …

WebHigh-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent … on the line youtubeWebApr 21, 2016 · Counter current chromatography (CCC) is a liquid chromatography technique that uses two immiscible liquid phases and no solid support. 2. One liquid acts as the stationary phase and the other as the mobile phase. 3. In Dual Flow CCC/CPC both liquid phases are flowing, as would be common in counter current process extractors. 4. ioof agmWebHigh-speed countercurrent chromatography Y. Ito Nature 326 , 419–420 ( 1987) Cite this article 267 Accesses 37 Citations Metrics Abstract Support-free high-speed … on the line مترجمWebAn efficient high-speed counter-current chromatography method for the preparative separation of potential antioxidants from Paeonia lactiflora Pall. combination of in vitro … onthelistWebHigh-speed counter-current chromatography (HSCCC) is a liquid-liquid chromatographic technique based on solvent partition that eliminates irreversible adsorption of samples onto the solid separation materials and produces excellent sample recoveries [10]. Thus, this method has been widely used for ioof apps storeWebIn this study, eight phenolic compounds were obtained from the over-ground parts of the Aconitum pendulum ( A. pendulum) Busch through an efficient high-speed counter-current chromatography （HSCCC）method. First, the column chromatography for macroporous resin was used for the treatment of crude extract of over-ground parts of A. pendulum … ioof apraWebJan 7, 2024 · High-speed counter-current chromatography (HSCCC) was first developed in the early 1980s . It is a kind of chromatographic technology based on the theory of liquid … ioof announcements