Webb20 sep. 2024 · It is necessary to develop a safe and efficient siRNA delivery vector with high anti-bacterial capability. Here, three multifunctional polymeric hybrid micelles … WebbEvery recombinant vector was linearized with HindIII and cloned into the pHM1 vector for genetic complementation. The hpa1pthXo1 double complementary vectors were constructed using two steps. First, the hpa1 sequence that was linked to its own promoter was cloned into pHM1 between the PstI and KpnI sites.
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Webb1 apr. 2007 · The broad host-range vector pHM1 (Yang et al., 2000) was used in complementation experiments. Concentrations of the antibiotics used were as follows: … WebbBasic Vector Information; Vector Name: pHM1: Antibiotic Resistance: Spectinomycin/Streptomycin Length: 12702 bp: Type: Cosmid vector Source: Gu K, Yang … poop illustration cute
Domain Dissection of AvrRxo1 for Suppressor, Avirulence and
Webb1 maj 2002 · An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent … Webb1 dec. 2016 · The complementing clone (pglA-/pHM1+ pglA) restored the polygalacturonase activity while the empty vector control strain (pglA-/pHM1) did not … Webb12 juli 2024 · Taq polymerase ReadyMix (KAPA Biosystems, Wilmington, MA, USA) was used for all screening purposes. For cloning in the pHM1 vector (Innes et al., 1988), primers as listed in Table S2 were used for amplification of the xopX gene and its 14-3-3 protein-binding motif mutants using Phusion polymerase (Thermo Fisher Scientific). poop illustration